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Esterization of the testosterone molecules provides for a sustained (but non-linear) release of testosterone from the injection depot into the blood plasma. This results in a significant increase in the production of androgens (testosterone, free testosterone) and the inhibition of the metabolism of other androgens. Testosterone is responsible for the increased muscular growth of both males and females, particularly in the older animals. The testosterone system has been extensively described for both males and females. The blood tests and the metabolic tests are performed on various groups of rodents (e.g., male and female C57BL/6J rats, and rats over 8 weeks old). The biochemical profiles of the rats are compared. The hormonal profile is assessed by calculating the free testosterone excretion (basal serum testosterone and free testosterone in serum) in the following steps: Measure testosterone in a testicular oil at an elevated concentration (∼10 nmol/l) for a 10-day period, and then immediately measure the free testosterone concentration of the testis (using an immunoassay). The testosterone excretion in the skin is determined by measuring the testosterone levels in the blood of males and females, and using the same methodology. Free testosterone determination is the first step of the blood test and the measurement of the serum testosterone excretion is performed when it reaches a plateau level. The metabolic profile is assessed in the following steps: The hepatic insulin secretory parameters are analysed, as are the hepatic glucose and triglyceride levels. The urinary glucose and nitrogen excretion is measured in the rats at a concentration of 100 mg/l and after 2-4 weeks and are determined from the glucose and nitrogen excretion, respectively, with the use of commercially available metabolic analyzers. To summarize, the hormonal profile of the rats used for the present study was as follows: The blood test, in the males, results in significantly lower testosterone excretion relative to control males. On the other hand, the excretion of gonadotropins is significantly increased in the control males; and the urinary glucose and nitrogen excretion is significantly decreased in the testis, similar to the other parameters analysed. The metabolic profile and blood test results showed a substantial increase in the production of free testosterone in males (testosterone levels of 10–20 nmol/l) and an increasing effect on the production of LH in females (testosterone levels of 2–4 nmol/l) compared to control animals. The increase in LH resulted in a significant increase of the sex-hormone-binding globulin (HBP) in serum. Similar articles:
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